Understanding the diverse cranberry measurement techniques is crucial for ensuring the effectiveness of your supplement choice.

What’s the best approach to gauge cranberry PACs effectively?

Various methods are employed globally, each yielding results that differ, sometimes by a factor of 1 to 5. Consequently, comparing PAC amounts using diverse testing techniques can be perplexing. Fortunately, there’s a novel testing method offering more uniform outcomes for both soluble and insoluble PACs in cranberry juice, powder, and whole fruit powders. This method is known as the 4-(dimethylamino)cinnamaldehyde (DMAC) assay.

Challenges in Measuring PACs with the DMAC Assay:

The DMAC Assay, based on the reaction between DMAC and the terminal units of PACs, is widely employed for quantifying total PAC content. However, its accuracy is contingent upon the choice of appropriate standards used for calibration. Traditionally, synthetic Procyanidin A2 and B2 have served as standards due to their structural similarity to naturally occurring PACs. Nevertheless, recent studies have questioned the adequacy of these standards, advocating for the utilization of c-PACs (cranberry-derived PACs) for improved accuracy.

One of the primary challenges with using Procyanidin A2 and B2 lies in their synthetic nature, which may not precisely mimic the PACs found in natural sources. Consequently, calibration based on these standards may underestimate the true PAC content in cranberry products, thereby compromising the efficacy of UTI prevention strategies. In contrast, employing cranberry derived c-PACs as standards offers a more representative and accurate measure of natural PACs present in cranberries, enhancing the reliability of DMAC Assay results.

Simply put, by utilizing c-PAC as a standard in the DMAC assay, researchers can account for variations in PAC structure and bioavailability, thus enhancing measurement precision. This approach ensures that study outcomes better reflect the biological activity of PACs in UTI prevention.

What preferred method (DMAC/A2 or DMAC/c-PAC) should I look for when choosing a cranberry supplement?

The short answer: DMAC/c-PAC. However, you don’t have to take our word on this, just have a look at what the scientists say in published scientific literature which can be verified by anyone that has a computer:

“Thus, the use of monomers and procyanidin dimers (procyanidin A2) as DMAC standards to estimate the content of PAC oligomers of higher DP (degree of Polymerization) may be inaccurate and may greatly underestimate PAC content.”

  1. Rodrigo P. Feliciano,† Michael P. Shea,§ Dhanansayan Shanmuganayagam,§ Christian G. Krueger,§,# Amy B. Howell,⊗,# and Jess D. Reed*,§,#, Comparison of Isolated Cranberry (Vaccinium macrocarpon Ait.) Proanthocyanidins to Catechin and Procyanidins A2 and B2 for Use as Standards in the 4-(Dimethylamino)cinnamaldehyde Assay, J. Agric. Food Chem. 2012, 60, 4578−4585

“… the cranberry marketplace has recognized the utility of the c-PAC reference standard to provide more accurate quantification of PACs than the procyanidin A2 dimer.”

  1. Erica R. Gullickson,† Christian G. Krueger,*,†,‡ Andrew Birmingham,‡ Michael Maranan,‡ and Jess D. Reed†,‡, Development of a Cranberry Standard for Quantification of Insoluble Cranberry (Vaccinium macrocarpon Ait.) Proanthocyanidins, J. Agric. Food Chem. 2020, 68, 2900−2905

“…Current use of procyanidin A2 as a standard leads to an underestimation of PACs content in certain cranberry products, especially those containing higher molecular weight PACs (i.e. insoluble PACS)…..Use of the c-PAC standard to quantify PAC content in cranberry samples resulted in values that were 2.2 times higher than those determined by procyanidin A2. Increased accuracy is critical for estimating PAC content in relationship to research on authenticity, efficacy, and bioactivity, especially in designing clinical trials for determination of putative health benefits.”

  1. Rodrigo P. Feliciano,† Michael P. Shea,§ Dhanansayan Shanmuganayagam,§ Christian G. Krueger,§,# Amy B. Howell,⊗,# and Jess D. Reed*,§,#, Comparison of Isolated Cranberry (Vaccinium macrocarpon Ait.) Proanthocyanidins to Catechin and Procyanidins A2 and B2 for Use as Standards in the 4-(Dimethylamino)cinnamaldehyde Assay, J. Agric. Food Chem. 2012, 60, 4578−4585

“The use of c-PAC in the DMAC assay showed a more pronounced effect on PAC estimation because cranberry powders and juices were underestimated up to 47% compared to procyanidin A2 results.”

  1. Rodrigo P. Feliciano,† Michael P. Shea,§ Dhanansayan Shanmuganayagam,§ Christian G. Krueger,§,# Amy B. Howell,⊗,# and Jess D. Reed*,§,#, Comparison of Isolated Cranberry (Vaccinium macrocarpon Ait.) Proanthocyanidins to Catechin and Procyanidins A2 and B2 for Use as Standards in the 4-(Dimethylamino)cinnamaldehyde Assay, J. Agric. Food Chem. 2012, 60, 4578−4585

“The adoption of c-PAC standard should be considered as an improvement over the use of ProA2 for accurate quantification of cranberry PAC.”

  1. Christian G. Krueger a,b,*, Nathan Chesmore a, Xin Chen b,Jessica Parker b, Christina Khoo c, Jannie P.J. Marais c,Dhanansayan Shanmuganayagam b, Peter Crump b, Jess D. Reed a,b, Critical reevaluation of the 4-(dimethylamino)cinnamaldehyde assay:Cranberry proanthocyanidin standard is superior to procyanidin A2 dimer for accurate quantification of proanthocyanidins in cranberry products, Journal of Functional Foods 22 ( 2 0 1 6 ) 13–19, 2016 Elsevier Ltd. All rights reserved.

“The use of c-PAC as a standard improves the accuracy of PAC quantification when compared to the use of the procyanidin A2 standard.”

  1. Rodrigo P. Feliciano,† Michael P. Shea,§ Dhanansayan Shanmuganayagam,§ Christian G. Krueger,§,# Amy B. Howell,⊗,# and Jess D. Reed*,§,#, Comparison of Isolated Cranberry (Vaccinium macrocarpon Ait.) Proanthocyanidins to Catechin and Procyanidins A2 and B2 for Use as Standards in the 4-(Dimethylamino)cinnamaldehyde Assay, J. Agric. Food Chem. 2012, 60, 4578−4585

“The widely used methods such as the 4-(dimethylamino) cinnamaldehyde (DMAC) assay may give inaccurate results when comparatively simple single compounds, such as a procyanidin A2 (ProA2) dimer, are used as a standard”

  1. Christian G. Krueger a,b,*, Nathan Chesmore a, Xin Chen b,Jessica Parker b, Christina Khoo c, Jannie P.J. Marais c,Dhanansayan Shanmuganayagam b, Peter Crump b, Jess D. Reed a,b, Critical reevaluation of the 4-(dimethylamino)cinnamaldehyde assay:Cranberry proanthocyanidin standard is superior to procyanidin A2 dimer for accurate quantification of proanthocyanidins in cranberry products, Journal of Functional Foods 22 ( 2 0 1 6 ) 13–19, 2016 Elsevier Ltd. All rights reserved.

In the quest to combat UTIs effectively, harnessing the potential of PACs holds great promise. Accurate measurement of both soluble and insoluble PAC levels is critical for understanding their combined efficacy in UTI prevention. Moreover, utilizing c-PAC as a standard in the DMAC assay ensures measurement accuracy, thereby enhancing the reliability of research findings. By embracing dual-action PAC supplements alongside with D-Mannose (both at their clinically effective doses), individuals may benefit from comprehensive protection against UTIs, ultimately improving urinary tract health and reducing the burden of recurrent infections.

IMPORTANT NOTE: Be cautious of assertions from certain proanthocyanidin supplement manufacturers who claim superiority in their measurement methods without referencing accessible published literature. These are the same companies that may inaccurately state higher PAC content than what is indicated on their Health Canada license and packaging.
 
It’s crucial to remember that products subjected to comprehensive evaluation for quality, safety, and efficacy by Health Canada typically bear a Natural Product Number (NPN). Conversely, products lacking an NPN on their label or packaging likely haven’t undergone thorough Health Canada evaluation and may not meet stringent standards. Essentially, the Health Canada NPN guarantees that you receive precisely what is stated on the label and what you’ve paid for.

“Why should you settle for less?”

UNIQUE FORMULATION: URIEXO’s unique formulation with D-Mannose (2000 mg/day) & Proanthocyanidins (36 mg/day PACs) helps prevent Urinary Tract Infections (UTIs).

CLINICAL STRENGTH: URIEXO is formulated with not one but two clinically active ingredients at the highest clinically effective dose allowed by Health Canada.

PUBLISHED DATA: The data behind URIEXO has been published in Scientific Journals..

COST EFFECTIVE: URIEXO is almost ½ price compared to combining other brands containing just one active ingredient.

BONUS: Free UTI test strip included.

URIEXO:

  • Helps prevent Urinary Tract Infections and its symptoms:
    • Painful or difficult urination
    • Frequent urination
    • Urgency to urinate
    • Suprapubic/pelvic pain
  • Two Medicinal Ingredients:
    • D-Mannose (2000 mg/day)
    • PACs, sourced from cranberry (36mg/day)
  • Antibiotic-Free
  • Easy to Swallow Capsules

Developing a daily routine with URIEXO is the secret to preventing and eliminating UTIs, once and for all.

Uriexo Vegicaps – For Urinary Tract Infection (UTI), Antibiotic-Free, D-Mannose & Proanthocyanidins (PACs)

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